HPLC Refractive-Index (RI) Detector  

RI detector measures change in reflex index. A glass cell is divided into two chambers (cells). The effluent from LC column flow through the "sample cell", while other cell called "reference cell" is filled with only mobile phase. When the effluent going through the sample cell does not contain any analyte, the solvent inside both cells are the same (Figure 1A). When a beam is irradiate on the cells, the observed beam will be straight in this case. However, in a case the effluent contains any components other than mobile phase; bending of the incident beam occurs due to the reflex index difference between the two solvents (Figure 1B). By measuring this change, the presence of components can be observed.

RI detector has lower sensitivity compared to UV detector, and that's the main reason

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Shimadzu Refractive Index LC Detector 20A (RID-20A)  

Inheriting the stability and extensibility that are the strengths of the Prominence series, the new Shimadzu Refractive Index LC Detector 20A (RID-20A) model of differential refractive index detector is designed with a new reference-cell auto-purge feature and validation support function.

Specifications :

 
Pressure Relief Valve :
The RID-20A incorporates various safety features. Its maximum pressure is five times that of former Shimadzu products and, as a standard feature, it incorporates a sensor that detects leakage from the cell unit. For extra safety, a pressure relief valve that prevents problems related to back-pressure irregularities is also available as an option.

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High Performance Liquid Chromatography Detectors ( HPLC Detectors)  

1. HPLC UV, VIS, and PDA Detectors
2. HPLC Refractive-Index Detector
3. HPLC Evaporative Light Scattering Detector
4. HPLC Multi-Angle Light Scattering Detector
5. HPLC Mass Spectrometer
6. HPLC Conductivity Detector
7. HPLC Fluorescence Detector
8. HPLC Chemiluminescence Detector
9. HPLC Optical Rotation Detector
10.HPLC Electro Chemical Detector
The actual separation of each component in the sample is carried inside a column; however this separation needs to be "collected" for us to be able to see it. The detectors are used for this purpose. The separated coponents are monitored and expressed electronically. There is no universal detector that can monitor all compounds and there are many detectors used for LC analysis. Some are listed below.


Type
Common Abbreviation

Ultra Violet
UV

V

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Agilent 1290 Infinity II Refractive Index LC Detector  

The 1290 Infinity II Refractive Index Detector is equipped with an ultralow dispersion microflow cell, which significantly reduces run times for higher sample throughput and improved resolution. Lower solvent consumption means much lower cost of analysis. A high-performance detector of choice for accurate, reproducible, routine analysis of polymers and other compounds that aren’t detectable by UV.
Features Of Agilent 1290 Infinity II Refractive Index LC Detector:
Shorter run times – for considerably higher sample throughput.
Ultralow dispersion – for improved sample definition and resolution.
Reduced solvent consumption – for significant savings in analysis cost.
Ideal tool for polymer analysis – consistent molecular weights, micro or analytical scale.
Excellent sensitiv

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What Is Evaporative light scattering detector (ELSD)?  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds that do not absorb UV radiation and therefore cannot be detected by UV detectors, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols.ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI)./wikipedia

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1290 HPLC  

The Agilent 1290 Infinity LC system provides the highest levels of speed, resolution, flexibility and sensitivity for any LC and LC/MS application. 

 

Used Agilent 1290 Infinity Series HPLC System

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agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1290 HPLC , Agilent 1290 LC System,Used Agilent 1290, Infinity Series, HPLC System This ...

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1290 Infinity Series HPLC

hplcpumps.hplchplc.com/list/1290+Infinity+Series+HPLC.html

 

 Used Agilent 1290 Infinity Series HPLC System · Download Hplc Agilent Technologies1290 Infinity II LC System Brochure and Guide. Downloa

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HPLC Column | Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column  

ZORBAX Carbohydrate Analysis Columns are reproducible, efficient, and flexible. These columns use ZORBAX porous silica microsphere technology. Silica manufacture, bonding and packing are all performed in our ISO9001 facilities. ZORBAX Carbohydrate Analysis Columns can handle high volume injections as much as 50 µL on a 4.6 x 150 mm column.
Features Of Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column:
Reproducible
Efficient – uses ZORBAX porous silica microsphere technology; silica manufacturing, bonding and packing are all performed in Agilent's ISO 9001 facilities
Flexible – can handle high volume injections – as much as 50 μL on a 4.6 x 150 mm column
Recommended for use with refractive index detectors (RID)

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Waters Breeze 2 HPLC  

The Breeze™ 2 HPLC System delivers technology and performance in an affordable, compact, and user-friendly system platform. Complete with software, pump, detector and injector, the system comes pre-configured for different levels of HPLC operational needs.
From teaching purposes to daily analytical work, the Breeze 2 HPLC System integrates simplicity, sensitivity, accuracy, and reliability. It is ideal for any organization seeking a quality HPLC platform with limited budget and chromatography experience including university laboratories, government laboratories, or start-up companies.
The Waters® Breeze 2 HPLC System delivers routine analyses and robust performance day after day, providing the  onfidence you need to get the job done. Used in laboratories worldwide, chromatograp

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Evaporative Light Scattering Detector (ELSD)  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds where UV detection might be a restriction and therefore compounds does not very efficient absorb UV radiation, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols. ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI).

ELSD | Evaporative Light Scattering Detector

elsd.hplchplc.com/

 

... Scattering Detector. An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC).

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P

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Shimadzu Evaporative Light Scattering LC Detector LTII (ELSD-LTII)  

In the history of high-performance liquid chromatographs, which dates to the early 1960s, refractive index detectors (RI detectors) have often been used as general-purpose detectors. RI detectors enable the detection of components that do not possess UV absorbance and give a proportional relationship between the heights of detected peaks and the quantities of detected components. So, in comparison with absorbance detectors (UV detectors), they offer advantages such as the ability to ascertain unknown component quantities and obtain molecular weight distributions for macromolecules. On the other hand, they also have various disadvantages. For example, they cannot be used for gradient analysis, the baselines they produce are susceptible to the influence of fluctuations in the ambie

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Chromatography terms / analyte / Analytical chromatography / bonded phase / chromatogram /...  

The analyte is the substance to be separated during chromatography. It is also normally what is needed from the mixture.
Analytical chromatography is used to determine the existence and possibly also the concentration of analyte(s) in asample.
A bonded phase is a stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing.
A chromatogram is the visual output of the chromatograph. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture.
 Plotted on the x-axis is the retention time and plotted on the y-axis a signal (for example obtained by a spectrophotometer, mass spectrometer or a variety of other detectors) corresponding to the response creat

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