1290 HPLC  

The Agilent 1290 Infinity LC system provides the highest levels of speed, resolution, flexibility and sensitivity for any LC and LC/MS application. 

 

Used Agilent 1290 Infinity Series HPLC System

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agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1290 HPLC , Agilent 1290 LC System,Used Agilent 1290, Infinity Series, HPLC System This ...

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1290 Infinity Series HPLC

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 Used Agilent 1290 Infinity Series HPLC System · Download Hplc Agilent Technologies1290 Infinity II LC System Brochure and Guide. Downloa

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1100 HPLC  

Introduced in 1995, Agilent's 1100 series HPLC system is considered the world's most popular HPLC. They are incredibly robust machines that can last a long time. Finding a used Agilent 1100 on LabX is easy and maintenance for older machines consists of changing seals, filter solvents, lamps, and the odd part in the vacuum degasser. Parts from the Agilent 1050 are often shared with the Agilent 1100 which make it easy to maintain.

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Agilent Technologies 1100 Series

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Used Agilent 1100 Series HPLC with Multiple Wavelength Detector System. 1100 hplc,agilent hplc,hplc system,used hplc,used agilent hplc. HPLC System with ...

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Used Agilent 1100 S

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Agilent HPLC 1260  

The Agilent 1260 Infinity HPLC-Chip/MS system is a re-useable microfluidic chip-based technology for high sensitivity nanospray LC/MS. The Agilent 1260 replaced the Agilent 1200 and delivers results faster to save time and money. 

Agilent Technologies 1260 Series

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agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1260 HPLC , Agilent 1260 LCSystem,Used Agilent 1260, Infinity Series, HPLC System,DAD ...

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agilent 1260 hplc

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This Agilent 1260 HPLC included: Binary pump with switch valve 1260 Binary Pump Thermostatted Column Compartment (TCC SL+)

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Agilent HPLC  

Find great deals on HPLCHPLC.COM for Used and Refurbished  Agilent HPLC in Analytical Lab Instruments. Shop with confidence. 





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agilent 1200 hplc


hplc.hplchplc.com/list/agilent+1200+hplc.html
 
This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings: G1379B Degasser with Tray G2226A ...
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Agilent Technologies 1260 Series


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agilent hplc,agilent liquid chromatography,Agilent 1290,Agilent 1260 HPLC , Agilent 1260 LCSystem,Used Agilent 1260, Infinity Series, HPLC System,DAD ...
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USED AGILENT HP 1200 HPLC Series SYSTEM


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used

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Agilent HPLC 1200  

The Agilent 1200 Series HPLC System was introduced in 2010 with a modular design allowing users to define a configuration ideally suited to meet their HPLC and UHPLC applications and requirements. 

agilent 1200 hplc

hplc.hplchplc.com/list/agilent+1200+hplc.html

This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings: G1379B Degasser with Tray G2226A ...

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USED AGILENT HP 1200 HPLC Series SYSTEM

hplccolumn.hplchplc.com/page-461164.html

used hplc,agilent 1200, 1200 hplc,used agilent 1200 hplc,on sale hplc. We offer an Agilent Technologies 1200 Series HPLC System in excellent working ...

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waters hplc  

Whatever your application, Alliance® HPLC, BreezeTM 2 HPLC, and AutoPurificationTM systems offer you proven solutions for all of your HPLC requirements - for today and the future. These systems are synonymous with dependable, routine performance and versatility.

Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance HPLC family meets the rigorous requirements of routine chromatography and the performance standards of new-product research and method development.

Breeze 2 HPLC Comes with a pump, detector, injector, and is pre-configured for different levels of HPLC. It includes Breeze Software, an intuitive interface that is easy to set-up, learn, and use.
waters


waters.hplchplc.com/list/waters.html
 
Waters Alliance HPLC THAT WORKS AS HARD AS YOU

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Chromatography terms / analyte / Analytical chromatography / bonded phase / chromatogram /...  

The analyte is the substance to be separated during chromatography. It is also normally what is needed from the mixture.
Analytical chromatography is used to determine the existence and possibly also the concentration of analyte(s) in asample.
A bonded phase is a stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing.
A chromatogram is the visual output of the chromatograph. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture.
 Plotted on the x-axis is the retention time and plotted on the y-axis a signal (for example obtained by a spectrophotometer, mass spectrometer or a variety of other detectors) corresponding to the response creat

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Shimadzu Refractive Index LC Detector 20A (RID-20A)  

Inheriting the stability and extensibility that are the strengths of the Prominence series, the new Shimadzu Refractive Index LC Detector 20A (RID-20A) model of differential refractive index detector is designed with a new reference-cell auto-purge feature and validation support function.

Specifications :

 
Pressure Relief Valve :
The RID-20A incorporates various safety features. Its maximum pressure is five times that of former Shimadzu products and, as a standard feature, it incorporates a sensor that detects leakage from the cell unit. For extra safety, a pressure relief valve that prevents problems related to back-pressure irregularities is also available as an option.

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Used Agilent 1200 Hplc system with Degasser,Bin pump ,Hip-als ,MWD,TCC  

The binary pump comprises two identical pumps integrated into one housing. It provides gradient generation by high-pressure mixing The binary pump is based on a two-channel, dual-piston in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by two pump assemblies which can generate pressure up to 400 bar.

Each channel comprises a pump assembly including pump drive, pump head, active inlet valve which has a replaceable cartridge, and outlet valve. Both channels are connected in a low-volume mixing chamber which is connected by a capillary coil to a damping unit and a mixer. A purge valve including a PTFE frit is fitted at th

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HPLC (high performance liquid chromatograph ) Instrumentation  

Main components in an HPLC system include the solvent reservoir, or multiple reservoirs, a high-pressure pump, a column, injector system and the detector.

The reservoir holds the solvent, which is referred to as the mobile phase because it moves. There are usually a minimum of two reservoirs in a system, with each holding up to 1000 cc of solvent and usually fitted with a gas diffuser through which helium can be bubbled. A pump is used to generate a specified flow of the mobile phase. Although manual injection of samples is still possible, most HPLCs are now fully automated and controlled by computer. The injector, or auto sampler, introduces the solvent into a phase stream that carries the sample into the high pressure (up to 400 bar) column, which contains specific packing material nee

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What Is High Performance Liquid Chromatography (HPLC)?  

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

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principle of hplc:High Performance Liquid Chromatography (HPLC)  

High performance liquid chromatography (HPLC) has become a very versatile and powerful separation and analytical method over the years. It is an advanced form of liquid chromatography (LC).
Instead of introducing the solvent into the column and allowing it to drip down under the influence of gravity, in HPLC the sample is forced through the column under high pressures of nearly 400 atm, resulting in faster and more efficient separation.
This technique is also called high pressure liquid chromatography.

The Principle of HPLC
HPLC follows the same basic principle as chromatography. Different components in the sample have varying affinities to the adsorbent material. This causes a difference in the flow rate for each component which leads to their separation as they come out of the column.

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What is High Performance Liquid Chromatography(HPLC)?  

HPLC stands for High Performance Liquid Chromatography.
Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography. The technique has found immense scope of applications in both academic and industrial laboratories requiring identification and quantification of mixtures of organic compounds.
Why not start with a short video?
Are you new to High Performance Liquid Chromatography? Simply spend about 2 to 3 min on the introductory video giving you an insight into what constitutes a HPLC system.
 
http://lab-training.com/hplc/
source:lab-training.com

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Shimadzu Nexera XR  

The Most Accessible UHPLC Available 
Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods.
Expanding the Possibilities of UHPLC :


Superb Functionality

Accuracy and reproducibility of solvent delivery and injection volume are particularly important performance aspects. Shim

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Shimadzu Prominence nano  

The Most Accessible UHPLC Available 
Developed with expandability and compatibility in mind, the Nexera XR ultra high performance liquid chromatograph enables more customers to make use of high-speed, high-resolution systems. Configure the optimal system to meet the specific analysis objective by selecting from among a wide range of highly accurate and reliable modules. The next milestone in the evolution of liquid chromatography, the Nexera XR promises to become an indispensable tool in laboratories in a variety of fields, including pharmaceuticals, biochemistry, chemistry, environmental, and foods.
Expanding the Possibilities of UHPLC :


Superb Functionality

Accuracy and reproducibility of solvent delivery and injection volume are particularly important performance aspects. Shim

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High performance liquid chromatography (HPLC)  

High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). The sample is carried by a moving carrier gas stream of helium or nitrogen. HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion. Because of this versatility, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals.

Sample retention time will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the s

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Waters ACQUITY UPLC H-Class System  

With dispersion of less than 10 μL, the ACQUITY UPLC H-Class System delivers the highest resolution of any quaternary LC system in the market. Achieving true UPLC performance requires that system dispersion (or extra-column band spread) be matched to the peak widths associated with narrowbore columns packed with sub-2-μm particles.  While other systems might be designed for high pressure, it doesn’t mean they're designed to deliver the sharp peaks associated with UPLC performance and the ACQUITY UPLC H-Class System.
Highest resolution of any quaternary LC system

Improve your laboratory’s efficiency by scaling your methods to UPLC Technology
Use UPLC’s unmatched resolution to get better characterization of your complex samples
Take advantage of tools that simplify and str

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What Is Column chromatography?  

For more details on this topic, see Column chromatography.
Column chromatography is a separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open tubular column). Differences in rates of movement through the medium are calculated to different retention times of the sample.
In 1978, W. Clark Still introduced a modified version of column chromatography called flash column chromatography (flash).The technique is very similar to the traditional column chromatography, except for that the

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Troubleshooting HPLC- Tailing Peaks  

As in the previous posts for this series, these suggestions assume that you are working with an established method and had successful results in the past. If a method is rugged, it is developed to minimize tailing by using a stationary column phase that is base-deactivated as well as appropriate modifiers and pH adjustment in the mobile phase as needed.  Keep in mind that if the method is not as rugged as it should be, change(s) in the method might be advised if tailing continues to be an ongoing problem.

With troubleshooting, as always, it is critical to note when the change occurred and how it might correlate to changes in the system.  Equally important is to change one thing at a time to identify the source(s) of difficulty. The following are examples of things that could contribute

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principle of hplc:How Does High Performance Liquid Chromatography Work?/what is Detector,Chromatogram?  

How Does High Performance Liquid Chromatography Work?

The components of a basic high-performance liquid chromatography [HPLC] system are shown in the simple diagram in Figure E.
A reservoir holds the solvent [called the mobile phase, because it moves]. A high-pressure pump [solvent delivery system or solvent manager] is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute. An injector [sample manager or autosampler] is able to introduce [inject] the sample into the continuously flowing mobile phase stream that carries the sample into the HPLC column. The column contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.

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chromatography  

Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.

Chromatography

chromatography.hplchplc.com/ 
 







Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.


Partition Chromatography


chromatography.hplchplc.com/page-461157.html
 
Partition chromatography was one of the first k

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Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes  

Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
We occasionally get calls or emails from folks that are seeing lower than expected response for peaks of interest. This may be a matter of method development if you have not done this analysis previously. Suggestions in the post are provided assuming you are working with an established method and have had successful results over an extended period of time (until recently). These suggestions also assume that the response is low for quantitation standards as well as in prepared QC samples. If

ادامه مطلب  

Troubleshooting HPLC- Loss in Response for all Analytes  

Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
We occasionally get calls or emails from folks that are seeing lower than expected response for peaks of interest. This may be a matter of method development if you have not done this analysis previously. Suggestions in the post are provided assuming you are working with an established method and have had successful results over an extended period of time (until recently). These suggestions also assume that the response is low for quantitation standards as well as in prepared QC samples. If t

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Shimadzu RF-20A/RF-20Axs Fluorescence LC Detector  

The RF-20A/20AXS fluorescence detectors offer world-leading sensitivity and ease-of-maintenance. The RF-20AXS is a high-sensitivity model that incorporates a temperature-controlled cell with a cooling function as standard.

RF-20A / Standard model:
The RF-20A, which offers best-in-class sensitivity, features a water Raman S/N ratio of at least 1200, as well as excellent ease-of-use with such features as maintenance from the front panel and adoption of a long-life lamp.

RF-20AXS / Achieves World-Leading Sensitivity :
Offering world-class levels of sensitivity and easy maintenance, the RF-20AXS features a water Raman S/N ratio of at least 2000 and a temperature-controlled cell with a cooling function. This maintains a constant detector cell temperature, even if the room temperature

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Agilent 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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Hplc Agilent Technologies 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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Principle of HPLC:High Performance Liquid Chromatography (HPLC) : Principle, Types, Instrumentation and Applications  

Chromatography is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of variuos components. Chromatographic separations can be carried out using a variety of stationary phases, including immobilized silica on glass plates (thin-layer chromatography), volatile gases (gas chromatography

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